Histologically, sebaceous adenomas are benign neoplasms consisting of sebaceous cells arranged in nests forming acinar and duct-like structures. Results of the cyclic fatigue tests were analyzed by using a two-way analysis of variance (Minitab version 14.1; Minitab Inc, State College, PA). Plays everything - files, discs, webcams, devices, and streams. Promoter activity was normalized by dividing luciferase activity by SPAP activity. The test resulted in power of.
The study concluded that some important health behaviors in parents, such as tooth brushing habits are important determinants of these behaviors in their young children. So promoting parent knowledge and attitude could affect their children oral health behavior and status.
- These included caregivers’ oral health behaviors and demographic factors
- It is widely acknowledged that the behavior of parents affects their children’s health
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Majority of bacteriological media used in this study were purchased from Oxoid Co, UK, unless otherwise stated. Proteinase K and Dream Taq Green Master Mix 2X were purchased from Thermo Scientific Co, USA. Isopropanol, acetone, acetonitrile, toluene, methyl alcohol, and ethyl alcohol were purchased from Sigma, Co, USA. Lysozyme and agarose were purchased from Bio Basic Inc, Canada. DNA ladder (100 bp DNA Ladder H3 RTU (Ready-to-Use) was purchased from GeneDirex, Inc, Taiwan. Universal primers specific for 16S rDNA gene were synthesized by Bioneer Co, Korea.
Each factor was examined in two levels designated as −1 (low level) and +1 (high level). The design relies on the fact that each factor is studied in a low level in a set of ()/2 trials and in a high level in the other set ()/2 trials. All trials were carried out in triplicate in 250 mL Erlenmeyer flasks with a working volume of 100 mL broth. The averages of three readings were taken as the experimental response value.
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The statistical analysis was carried out using Minitab version 14.1 (Minitab (https://sarawakscale.com/activation-key/?patch=8290) Ltd. Application Lifecycle Management Integration Low-Code Development No-Code Development Mobile App Development Test Management UX. Analytics. Data were analyzed by Minitab statistical software version 16. Regression analysis was used to assess the relationship between dmft of children with some demographic variables and also parental oral health behaviors. More than 50% of all European children suffer a dental injury (DI), for which the emergency treatment is usually carried out by a general dentist. The Mass Deployment Package is required for a managed deployment of Minitab All languages are included. Thus, the purpose of this study was to.
Amira M. Embaby put the design of most laboratory experiments especially those concerned with statistical modeling and identified the producer bacterial strain via molecular approach. She was responsible for writing-revision of the whole paper, statistical analysis of data, scientific interpretation, and data discussion. Yasmin Heshmat carried out most of the laboratory experiments. Ahmed Hussein participated in data analysis, results interpretation, paper revision, and language editing. Heba S. Marey performed statistical analysis of RSM experiment especially canonical analysis.
Response surface methodology approach in the form of Box-Behnken design (BBD) was applied in this study to determine the optimal level of each key determinant that was identified by PBD. All forms of interactions among the independent variables are shown in this design. Each factor was deliberated in three levels denoted as −1 (low level), 0 (center level), and +1 (high level) through fifteen experimental runs. All trials were conducted in triplicate in 250 mL Erlenmeyer flasks with a working volume of 100 mL broth. The averages of three readings were considered as the response experimental values.
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Diverse mechanisms describing the bacteriocin mode of action were outlined but the cell membrane still remains the main target of these AMPs. The net positive charges of bacteriocins stimulate preferential binding of bacteriocins to the negatively charged bacterial cell membranes resulting in creation of multiple pores in the cell membranes [12–14]. In the context of bacteriocins as an adequate alternative to antibiotics against multidrug resistant pathogens along with a continuous urgent need for developing new antimicrobial agents, bacteriocins represent a promising inexhaustible source of new antibiotics [15, 16].
Influence of Finishing and Polishing Techniques and
Bacteriocins can antagonize the growth of closely related species (narrow spectrum) or across genera (broad spectrum). Their usage as potential weapons against human/animal/plant pathogens and food spoiler microorganisms has attracted the scientific attention in recent years [1, 5–8].
The present data exceedingly prompt applying directed evolution methodologies in order to improve the characteristics of the promising broad spectrum antimicrobial activity of BAC YAS 1 in the future work. In this context, improving thermostability of the BAC YAS 1 over a wide range of temperature degrees along with prolonged exposure times is an issue of a prime importance if commercial industrialization of BAC YAS 1 is being intended in the future. Rational protein design, one of the currently applied methodologies, could be a powerful tool to achieve this task that in turn necessitates the indispensable need to purify and study the crystalline structure of BAC YAS 1.
In a nutshell, employment of optimization strategies in bioprocesses particularly for bacteriocin industry is considered to be an essential step. It aims not only to minimize the cost encountered in medium compositions but also to maximize the yield as well. By the end of sequentially statistical optimization procedure, we could end up with appreciable levels of the bacteriocin (470 AU/mL) after 62 hrs of the growth of Bacillus sp. YAS 1 strain under optimized conditions against E. amylovora.
Use WinZip, the world's most popular zip file utility, to open and extract content from RAR files and other compressed file formats. It is the best data analysis software which we used for analyzing. Basically, more complex assignments will cost more than simpler ones. There were 585 boys and 564 girls. SUPER EASY (took me just about a half an hour to do) and the kids LOVE it! VoyForums Announcement: Programming and providing support for this service has been a labor of love since 1997.
Among the examined indicator strains, the most sensitive strains to BAC YAS 1 could be listed in the following order regarding the inhibition zone diameter: E. amylovora, L. innocua, Klebsiella sp, and S. epidermidis. Additionally, no antimicrobial activity of BAC YAS 1 could be detected against E. coli and E. faecalis. Regarding the influence of BAC YAS 1 on some environmental bacterial strains, the growth of only two strains belonging to the genus Bacillus (B. licheniformis SHG2 and B. subtilis AS1) was inhibited by BAC YAS1. Conversely, none of the tested fungal and yeast strains were influenced by BAC YAS 1 antimicrobial activity. Surprisingly, none of the tested lactic acid bacterial strains (Lactobacillus bulgaricus, L. casei, L. lactis, and L. reuteri) were affected by BAC YAS 1 antimicrobial activity as shown in Table 6.
It can be concluded from the study results that some important health behaviors in parents, such as tooth (https://sarawakscale.com/activation-key/?patch=4059) brushing habits and frequency of consumption of sweet foods, are important determinants of these behaviors in their young children. Children with high-educated mothers had lower plaque index than the others. So, promoting parents’ knowledge and attitude could affect their children oral health behavior and status.
Bacteriocins by definitions are antimicrobial agents and those that demonstrate wide spectrum antimicrobial activity against both Gram-negative and Gram-positive bacteria are of a prime importance as chemotherapeutic and prophylactic agents. BAC YAS 1 demonstrated a broad spectrum antimicrobial activity against both Gram-negative and Gram-positive bacteria as mentioned above.
This study assessed some characteristics and behaviors of parents that may affect oral health behaviors and oral health statuses of their children. There was a significant relationship between the history of having dental problems in parents and dmft index in their children. Several factors, such as neglecting oral health by parents and their inability to pay for health services and genetic factors, might interpret this relationship. Parental history of dental problems may show their consideration to oral health behaviors. A lot of factors may cause poor oral health status of parents and these factors may cause poor dental health (high dmft) in children. Some previous studies have also addressed this issue [6, 18].
Significance of the model was indicated by this value, while the model value reflects that the chance is only 1/7% that this model value could occur due to noise. Significance of coefficients was evaluated by looking at value of the estimate.
So, promoting parents’ knowledge and attitude could affect their children oral health behavior and status
Determining the onset of the genes encoding for bacteriocin is one of the prerequisite data in the agenda of bacteriocin production on an industrial scale. Thus, BAC YAS 1 production was monitored along with the growth of its producer strain Bacillus sp. YAS 1 in the bacteriocin production medium. The onset of BAC YAS 1 production was recorded to be at the late exponential phase and increased during the stationary phase of growth reaching its maximum after 62 hrs of cultivation. This finding was in disagreement with other reported findings [29, 38–41]. For instance, the production of bacteriocin (Bac14) by B. subtilis 14B started after a 24-h-lag phase and then increased exponentially and reached its maximum within 96 h cultivation . BAC YAS 1 production was not associated with the growth of Bacillus sp. YAS 1 strain because the maximum yield of BAC YAS 1 was achieved during the stationary phase. The present finding is partially in agreement with that of cerein from B. cereus where cerein could be traced at the beginning of stationary phase [1, 39]. On the other hand, the present finding was contradicted by Pal et al. and Banerjee et al. who affirmed that the bacteriocin of W. paramesenteroides DFR-8 and that of L. brevis FPTLB3 were growth-dependent since majority of the bacteriocin was traced during the exponential phase of growth. Activity of cerein 7 was detected in cultures of B. cereus Bc7 near the end of exponential phase and reached its maximum at the beginning of stationary phase . It seems that the onset of bacteriocins encoding genes varies widely not only among distally related species and genera but also among strains belonging to the same species which necessitates the indispensable need to explore the exact time at which each bacteriocin encoding gene turns on in each newly isolated producer strain.
Although the literature reported the production of bacteriocins from bacteria belonging to different genera and species such as lactic acid bacteria, Bacillus spp, Escherichia coli, and Vibrio spp, a few reports exist concerning the key determinants controlling the levels of the produced bacteriocins from these bacteria. Additionally, the bottleneck for bacteriocin production from most bacterial strains is the low yield. In this context, optimizing BAC YAS 1 production is the first step in the agenda of large scale production. Here, the production of BAC YAS 1 was affected by neither soluble starch nor initial pH of the production medium. The present finding is inconsistent with other findings of Zhong et al. , Pal et al. , and Kaur et al. , where the production of bacteriocin from B. cereus XH25, Weissella paramesenteroides DFR-8, and Pediococcus acidilactici BA28, respectively, was significantly influenced by soluble starch and initial pH of the production medium. However, present finding concerning the impact of yeast extract on BAC YAS1 was in good agreement with those of Zhong et al. and Meera and Devi who stated that yeast extract did exert significant effect on the productivity of a bacteriocin-like substance from B. cereus XH25 and probiotic lactic acid bacteria, respectively [28, 31]. Pertaining to the influence of glycerol on BAC YAS 1 productivity, it did not exhibit any significant consequence.
The relationship between plaque index of children with mother’s education and history of dental problems in parents was significant. This means that the presence of dental problems in parents may follow by some problems such as poor hygiene (high-plaque index) in their children. It can also show that high dental problems in parents may affect their consideration to hygienic behaviors of their children; so, healthy parents are more likely to have children with lower plaque index than unhealthy parents. This finding confirms the previous study findings .
Design of Experiments with MINITAB
Production of bacteriocin was monitored along with the growth of the producer isolate. Briefly, 100 mL of BYP broth was inoculated with 0/5 mL of an overnight grown culture. The newly inoculated broth was incubated at 37°C with agitation speed of 200 rpm (New Brunswick Incubator Shaker, USA). One mL samples were withdrawn from the culture each hour to measure the bacterial growth spectrophotometrically at 420 nm (UV-Vis Spectronic helios spectrophotometer, Thermo Scientific Co, USA). Then, the cell-free supernatants obtained via centrifugation at 7,000 rpm for 3 min (Mikro 200 Hettich, Germany) were kept at 4°C until their usage in well cut diffusion assay to determine the bacteriocin activity as mentioned above in Section 2/9.
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The objective of the present study is to isolate a bacteriocin producing bacterium with a wide spectrum antimicrobial property. The aim is extended to optimize the production of the produced bacteriocin through a sequential statistical approach. To the best of our knowledge, there exists a plethora of literature concerning characterization of bacteriocins but there exists a few reports pertaining to highlighting key determinants controlling the production of bacteriocins by their producers.